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Apr 19, 2024
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BME 730 - Introduction to Imaging and Optics (3 units)
Theory and practical aspects of microscope-based techniques currently utilized in research in both the life and physical sciences. (BME 730 and PHAR 730 are cross-listed; credit may be earned in one of the two.)
Units of Lecture: 3
Student Learning Outcomes:
Upon completion of this course:
1. Students will be able to demonstrate understanding of the physical properties of light and how it influences the optical properties and limit of systems used to image biological samples.
2. Students will be able to demonstrate how to prepare biological samples for histology, immunocytochemistry and immunohistochemistry.
3. Students will be able to demonstrate familiarity with optics, advanced microscopy techniques such as visible light, confocal, total internal reflection, multiphoton and super resolution fluorescence microscopy, atomic force microscopy and imaging.
4. Students will be able to recognize the principles underlying advanced spectral analysis of protein-protein interactions and co-localization, and molecular diffusion by Förster (FRET) and Bioluminescence (BRET) Resonance Energy Transfer, Fluorescence Recovery After Photobleaching (FRAP), Fluorescence Lifetime Imaging Microscopy (FLIM) and Fluorescence Correlation Spectroscopy (FCS).
5. Students will be able to recognize the principles and applications of non-invasive in vivo imaging by ultrasonography and Doppler imaging, and nuclear magnetic resonance imaging.
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