BCH 711 - Microscopy of Cellular Dynamics

(3 units)

Hands-on training on advanced fluorescence microscopy techniques for biological samples, including but not limited to plants, bacteria, yeast, insects, etc. as well as developing the capability to analyze big imaging data for publication.

Recommended Preparation: a basic knowledge of cell biology and optical physics (e.g., PHYS 152A, PHYS 181, BIOL 315).

Grading Basis: Graded
Units of Lecture: 1
Units of Laboratory/Studio: 2
Offered: Every Fall

Student Learning Outcomes
Upon completion of this course, students will be able to:
1. demonstrate in-depth understanding of modern advanced fluorescence microscopy techniques and existing biosensors detecting signal molecules.
2. design imaging experiment for detecting cellular signaling molecules using available biosensors for small signaling molecules or metabolites.
3. Through hands-on training in advance fluorescence microscopy techniques, demonstrate the skills of operating the existing microscope modules such as spinning disc confocal and Keyence microscopes and process big data for publication.
4. teach other researchers how to use fluorescence microscopes to detect small signaling molecules in various biological samples, including plants, microbes, insects, etc.

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